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35984 induced apparent hcec destruction  (ATCC)


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    ATCC 35984 induced apparent hcec destruction
    Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC <t>35984),</t> a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of <t>HCEC</t> viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.
    35984 Induced Apparent Hcec Destruction, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35984+induced+apparent+hcec+destruction/Staphylococcus+epidermidis+(Winslow+and+Winslow)+Evans/pmc12633770-80-1-0
    Average 97 stars, based on 2287 article reviews
    35984 induced apparent hcec destruction - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model"

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

    Journal: Translational Vision Science & Technology

    doi: 10.1167/tvst.14.11.21

    Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC 35984), a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of HCEC viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.
    Figure Legend Snippet: Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC 35984), a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of HCEC viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.

    Techniques Used: Fluorescence, Microscopy, Staining, Calcein AM Assay

    HCEC viability after exposure to 17 S. epidermidis strains at varying concentrations. ( A ) Nonpathogenic strains. ( B, C ) Pathogenic strains. Asterisks indicate a significant decrease in cell viability compared with the PBS control. * P < 0.05; ** P < 0.01; *** P < 0.001; *** P < 0.0001.
    Figure Legend Snippet: HCEC viability after exposure to 17 S. epidermidis strains at varying concentrations. ( A ) Nonpathogenic strains. ( B, C ) Pathogenic strains. Asterisks indicate a significant decrease in cell viability compared with the PBS control. * P < 0.05; ** P < 0.01; *** P < 0.001; *** P < 0.0001.

    Techniques Used: Control

    HCEC viability distinguished pathogenic from nonpathogenic S. epidermidis strains. ( A ) Heatmap illustrating HCEC viability following exposure to varying concentrations of 12 pathogenic and 5 nonpathogenic strains. Comparison of HCEC viability after exposure to ( B ) 104, ( C ) 105, ( D ) 106, ( E ) 107, and ( F ) 108 CFU/mL of pathogenic and nonpathogenic groups. ROC analysis of the diagnostic performance of HCEC viability at ( G ) 104, ( H ) 105, ( I ) 106, ( J ) 107, and ( K ) 108 CFU/mL in distinguishing the pathogenic S. epidermidis strains. ns, not significant. ** P < 0.01.
    Figure Legend Snippet: HCEC viability distinguished pathogenic from nonpathogenic S. epidermidis strains. ( A ) Heatmap illustrating HCEC viability following exposure to varying concentrations of 12 pathogenic and 5 nonpathogenic strains. Comparison of HCEC viability after exposure to ( B ) 104, ( C ) 105, ( D ) 106, ( E ) 107, and ( F ) 108 CFU/mL of pathogenic and nonpathogenic groups. ROC analysis of the diagnostic performance of HCEC viability at ( G ) 104, ( H ) 105, ( I ) 106, ( J ) 107, and ( K ) 108 CFU/mL in distinguishing the pathogenic S. epidermidis strains. ns, not significant. ** P < 0.01.

    Techniques Used: Comparison, Diagnostic Assay

    Related Articles

    Fluorescence:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.

    Microscopy:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.

    Staining:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.

    Calcein AM Assay:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.

    Control:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.

    Comparison:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.

    Diagnostic Assay:

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model
    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).. The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.The clinical strain isolated from an infectious keratitis caused a progressive decrease in HCEC density at concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 CFU/mL.



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    35984 induced apparent hcec destruction  (ATCC)
    97
    ATCC 35984 induced apparent hcec destruction
    Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC <t>35984),</t> a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of <t>HCEC</t> viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.
    35984 Induced Apparent Hcec Destruction, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/35984+induced+apparent+hcec+destruction/Staphylococcus+epidermidis+(Winslow+and+Winslow)+Evans/pmc12633770-80-1-0
    Average 97 stars, based on 1 article reviews
    35984 induced apparent hcec destruction - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC 35984), a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of HCEC viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.

    Journal: Translational Vision Science & Technology

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

    doi: 10.1167/tvst.14.11.21

    Figure Lengend Snippet: Establishment and optimization of a coculture model using HCECs and S. epidermidis . The model was developed using three representative S. epidermidis strains: a reference strain (ATCC 35984), a commensal isolate from a normal conjunctiva, and a clinical isolate from a patient with keratitis. ( A ) Time-course analysis showing phase-contrast images of HCECs cocultured with the three strains at 10⁷ CFU/mL for up to 72 hours. ( B ) Dose–response analysis showing phase-contrast images of HCECs after 24 hours of coculture with various concentrations (10⁴ to 10⁸ CFU/mL) of the strains. ( C ) Corresponding fluorescence microscopy images of HCECs stained with calcein AM (green fluorescence for live cells) to assess cell viability under the same dose–response conditions. ( D–F ) Quantification of HCEC viability from the calcein AM assay following 24-hour treatment with ( D ) ATCC 35984, ( E ) normal conjunctiva, and ( F ) keratitis strains. Scale bars , 100 µm. * P < 0.01; ** P < 0.01; *** P < 0.001; **** P < 0.0001; calcein AM, calcein acetoxymethyl ester; NC, normal conjunctiva; ns, no significance.

    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).

    Techniques: Fluorescence, Microscopy, Staining, Calcein AM Assay

    HCEC viability after exposure to 17 S. epidermidis strains at varying concentrations. ( A ) Nonpathogenic strains. ( B, C ) Pathogenic strains. Asterisks indicate a significant decrease in cell viability compared with the PBS control. * P < 0.05; ** P < 0.01; *** P < 0.001; *** P < 0.0001.

    Journal: Translational Vision Science & Technology

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

    doi: 10.1167/tvst.14.11.21

    Figure Lengend Snippet: HCEC viability after exposure to 17 S. epidermidis strains at varying concentrations. ( A ) Nonpathogenic strains. ( B, C ) Pathogenic strains. Asterisks indicate a significant decrease in cell viability compared with the PBS control. * P < 0.05; ** P < 0.01; *** P < 0.001; *** P < 0.0001.

    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).

    Techniques: Control

    HCEC viability distinguished pathogenic from nonpathogenic S. epidermidis strains. ( A ) Heatmap illustrating HCEC viability following exposure to varying concentrations of 12 pathogenic and 5 nonpathogenic strains. Comparison of HCEC viability after exposure to ( B ) 104, ( C ) 105, ( D ) 106, ( E ) 107, and ( F ) 108 CFU/mL of pathogenic and nonpathogenic groups. ROC analysis of the diagnostic performance of HCEC viability at ( G ) 104, ( H ) 105, ( I ) 106, ( J ) 107, and ( K ) 108 CFU/mL in distinguishing the pathogenic S. epidermidis strains. ns, not significant. ** P < 0.01.

    Journal: Translational Vision Science & Technology

    Article Title: Differentiating Ocular Pathogenic Staphylococcus epidermidis Isolates Using a Human Corneal Epithelial Cell Coculture Model

    doi: 10.1167/tvst.14.11.21

    Figure Lengend Snippet: HCEC viability distinguished pathogenic from nonpathogenic S. epidermidis strains. ( A ) Heatmap illustrating HCEC viability following exposure to varying concentrations of 12 pathogenic and 5 nonpathogenic strains. Comparison of HCEC viability after exposure to ( B ) 104, ( C ) 105, ( D ) 106, ( E ) 107, and ( F ) 108 CFU/mL of pathogenic and nonpathogenic groups. ROC analysis of the diagnostic performance of HCEC viability at ( G ) 104, ( H ) 105, ( I ) 106, ( J ) 107, and ( K ) 108 CFU/mL in distinguishing the pathogenic S. epidermidis strains. ns, not significant. ** P < 0.01.

    Article Snippet: ATCC 35984 induced apparent HCEC destruction at concentrations of 10 6 , 10 7 , and 10 8 CFU/mL in the phase-contrast set ( B).

    Techniques: Comparison, Diagnostic Assay